The current study showed that kisspeptin was significantly higher in PCOS compared to control (322.4 vs. 235.3 ng/L), and after performing ROC analysis, kisspeptin showed good ability to predict PCOS, with serum level ≥271.234 ng/L predicting PCOS; at this point, kisspeptin showed higher specificity (88.6%) and lower sensitivity (74.4%) with 81.6% accuracy. Here, KISS1 is linked to polycystic ovary syndrome.