We focused the current study on effects of a minute genetic difference, therefore we opted to use only males of a highly inbred model of metabolic syndrome to maximize the homogeneity of our control and experimental groups and avoid e.g., the potential effects of estrous cycle on gene expression etc. In a smaller-scale study involving female offspring of both SHR and SHR-Zbtb16 strains, we found that maternal HSD administration led to an increase in fasting insulinemia in both strains and, exclusively in SHR females, to an improvement of glucose tolerance (Skolnikova et al., 2020). This evidence concerns the gene CHST3 and metabolic syndrome.