To investigate the effect of IGSF9 on the aggressive phenotype, we transfected two different IGSF9 siRNAs (siIGSF9#1 and siIGSF9#2) into CNE-2 and 5-8F cells to down-regulate IGSF9 (Fig. 6a) and performed CCK8, wound-healing and invasion assays to evaluate whether regulation of IGSF9 expression affects the proliferation, invasion and migration of NPC cells. Here, IGSF9 is linked to nasopharyngeal carcinoma.