In addition, flow cytometry was performed to measure the proportion of MDSCs, Th and CTL in the peripheral blood and tumor tissues of the mice, which revealed that the proportion of MDSCs was decreased, while the proportion of Th and CTL were dramatically increased in the cells co-transfected with miR-21 antagomir and sh-NC compared to the cells co-treated with antagomir NC and sh-NC, whereas the opposite trends were observed in cells co-transfected with miR-21 antagomir and sh-RUNX1 in relation to cells co-treated with miR-21 antagomir and sh-NC. This evidence concerns the gene RUNX1 and neoplasm.