And we also found the risk score system negatively reflected the infiltration level of anti-tumor immune cells (e.g. CD8+ T cells, B cell naïve, and plasma cells) and positively correlated to the infiltration level of macrophages M2, indicating the presence of higher density of TLS in tumor tissues in patients with lower risk score in PC, and more thorough transformation of pro-inflammatory to anti-inflammatory in the TME of PC patients with higher risk score. The gene discussed is CD8A; the disease is pachyonychia congenita.