To investigate the role of PUF60 in RCC development, we knocked down PUF60 with its specific siRNA in 786-O (mutant) and Caki-1 (wild type) cells, and found that knockdown of PUF60 significantly inhibited the proliferation and clonogenicity in 786-O and Caki-1 cells (Fig. 4A, 4E [left]; 4B, 4F [left]). The gene discussed is PUF60; the disease is renal cell carcinoma.