To further validate the interaction of PUF60 and TERT promoter, the 5'-biotin labeled wild-type TERT promoter probe was incubated with nuclear proteins of RCC cell lines (786-O, Caki-1, ACHN and SN12C), the interacted proteins were pulled down by streptavidin-agarose beads, and PUF60 was detected by its specific antibody by western blot, confirming that there was indeed interaction between PUF60 and TERT promoter (Fig. 2G, upper panel). The gene discussed is TERT; the disease is renal cell carcinoma.