Immunostaining of F-actin with fluorescently labeled phalloidin revealed more enrichment of F-actin structure in control cells, such as membrane ruffles and stress fibers, compared with ABLIM1-KO cells (Figure 6A, B), suggesting that ABLIM1 promotes F-actin formation in HCC cells, consistent with previous reports 32-33. Here, ABLIM1 is linked to hepatocellular carcinoma.