Upon infection, Salmonella selectively induces certain WNTs (WNT3a, WNT6 and WNT9a) to promote ISC survival.31 Next, we infected tamoxifen-administered Il22Ra1fl/fl;Lgr5-EGFP-creERT2+ and littermate cre- mice with Salmonella. We found Il22Ra1fl/fl;Lgr5-EGFP-creERT2+ were highly susceptible to infection with 100% mortality 5 days post infection as well as elevated, albeit not significant, bacterial dissemination to the liver (Supplementary Fig. 5f, g). This evidence concerns the gene WNT3A and infection.