Unbiased analysis of the MYC targets V2 gene set revealed clusters correlated with the tumor histopathology, and adenocarcinoma-derived PDOs exhibited the high expression of distinct genes from adenoma-derived PDOs related to cellular proliferation, including CDK4, PLK1 and 4, MCM4, and MYC (Fig. 7C, Supplementary Fig. S3). This evidence concerns the gene CDK4 and adenoma.