SRI and neurodegenerative disease: We have then evaluated the effect of Sorcin expression on MAMs in HeLa cells, using split-GFP-based contact site sensor (SPLICS) engineered to fluoresce when organelles are in proximity50, and a PLA-based approach, to assess whether Sorcin overexpression, observed in neurodegenerative diseases models, not only alters ER calcium handling, but also affects ER-mitochondrial contacts (Fig. 9).