We first established and characterized an in vitro model of endothelial dysfunction by subjecting human umbilical vein endothelial cells (HUVECs) to high glucose (HG, 25 mM D-glucose) and TNFα (5 ng/mL, to mimic inflammation; H + T) for 3 and 7 days (abbreviated as H + T Days 3 and 7). This evidence concerns the gene TNF and endothelial dysfunction.