In this study, we demonstrated that PRMT5 is a coactivator to enhance the formation of β-catenin in the nucleus of laryngeal carcinoma cells, this signaling might be at least partly mediated by Wnt4, and the PRMT5/Wnt4 axis could be a new mechanism in laryngeal carcinoma development. This evidence concerns the gene WNT4 and laryngeal carcinoma.