Immunofluorescent staining results showed that the subcellular localization of β-catenin was mainly in the nucleus after overexpression of Wnt4 in PRMT5-depleted cells, which indicated that the β-catenin accumulation in the nucleus is necessary for PRMT5–Wnt4-induced laryngeal carcinoma metastasis (Fig. 5e). Here, PRMT5 is linked to laryngeal carcinoma.