In total, 2 × 106 laryngeal carcinoma cells (Tu212–PRMT5–Luc) that stably overexpressed PRMT5, generated by lentiviral transfection, and also stably expressed firefly luciferase, were inoculated into the footpads of nude mice (Fig. 3g). Here, PRMT5 is linked to laryngeal carcinoma.