In addition, Tschaharganeh et al. used the Alfp-Cre p53fl/fl animal model to specifically knock out P53, promote the dedifferentiation of mature hepatocytes into nestin-positive HPCs, and induce the formation of mixed HCC-ICCA by activating WNT and NOTCH pathway which affects multiple processes of normal cell morphogenesis, including the differentiation of pluripotent stem cells, cell apoptosis, and proliferation [86, 87]. This evidence concerns the gene TP53 and hepatocellular carcinoma.