Compared to MS-NASH mice on CD or WDF without CCl4, administration of CCl4 significantly reduced body weight (Fig. 1a), food (Fig. 1b) and caloric (Fig. 1c) intake, as well as dramatically elevated ALT (Fig. 1d) and AST (Fig. 1e) measured ~ 24 h after CCl4 dosing. This evidence concerns the gene GPT and metabolic dysfunction-associated steatohepatitis.