To understand their mechanistic impact, we tested all eleven cancer-associated mutations in all biochemical assays (Figure 4). Remarkably, the seven mutants that map to region 1, including the SuppH mutation that faces the post-HSA domain within region 1 (R685H), all conferred a moderate reduction in ATPase activity, while simultaneously increasing DNA translocation—and thus improve the coupling of ATP hydrolysis to DNA translocation (Figure 4A, and mutants H452Y and R685H in Figure 4B). The gene discussed is ALB; the disease is cancer.