CASP8AP2 and malignant colon neoplasm: Many of these genes have been reported as cytotoxic in other siRNA or CRISPR knockdowns [22, 45] and are presumably essential for the viability of both normal and cancer cells, e.g. the ribosomal protein, the polo kinases, DNA polymerases, centromere proteins and proteasome associated genes; however, some of the cytotoxic genes should be considered as potential targets for colon cancer therapeutics, e.g. CDK1 [46], CASP8AP2 [47] and tuftelin-1.