The study objectives included: (1) determining whether the selected sequence of the tryptic podocin peptide for LC-MS-MRM can be used for detecting podocin in horses; and (2) whether the species-specific ELISA test is useful for this purpose, (3) whether the chosen methods are sensitive enough to detect kidney dysfunction and glomerular injury, (4) whether the results of both methods correspond with one another, (5) whether the results correlate with the hematological and biochemical data. The gene discussed is NPHS2; the disease is injury.