Anticoagulant HS oligosaccharides were ineffective in acetaminophen-induced liver injury20; however, since liver IR involves thromboinflammation we included anticoagulant 12-mer-1 in our study to explore how anticoagulant activity and HMGB1 binding effected hepatoprotection in liver IR injury. This evidence concerns the gene HMGB1 and digestive system neoplasm.