Then, viral loads in JEV-infected SLC35B2, HS6ST1, B3GAT3, and GLCE KO cells and in WT PK-15 cells were measured at 18 hpi (hours post-infection) by plaque assay, which only using JEV at an MOI of 0.03 or 0.1 (Fig. 4b). This evidence concerns the gene GLCE and infection.