We performed ISH for TROLL-2 and TROLL-3 and IHC for WDR26 in 378 tumour specimens, consisting of 51 ovarian (Biomax TMA, including serous and non-serous adenocarcinomas), 73 colon (Biomax TMA), 55 lung (Biomax TMA, including adenocarcinomas and squamous cell carcinomas), and 199 melanoma cases (Biomax TMA and Moffitt TMA). Here, WDR26 is linked to serous adenocarcinoma.