sgRNAs specific for ORF45 (immediate early), K5 (early), ORF34 (early late), and K8.1 (late) lytic KSHV genes were transduced into endothelial cells expressing CRISPR-Cas9, and expression of the respective viral gene products was analyzed during lytic-stage infection (Figure 2A). The gene discussed is KRT5; the disease is infection.