Moreover, the protein levels of ALP, Runx2, OCN, and OPN were significantly increased in hFOB1.19-OM cells transfected with TUG1 compared with the vector group, while the levels were decreased in osteogenic differentiated hFOB1.19 cells introduced with si-TUG1 (Figure 4B). The gene discussed is SPP1; the disease is ocular melanoma.