We validated this sphingolipidomic profile using gene expression and Western blot analyses, which suggested that overexpression of key enzymes, SMPD3, SGPL, ASAH2 (ceramidase/sphingosine synthase) and S1P phosphatase (SGPP2) that tilt sphingolipid rheostat toward increased ceramide and decreased S1P are associated with IDHmut gliomas, while not within IDHWT. This evidence concerns the gene SMPD3 and central nervous system cancer.