In summary, our results show that cooperative effect of stearic acid on the TNF-α induced production of MIP-1α/CCL3 depends on TLR4-TRIF-TBK1-IRF3 signaling cascade which suggests an interesting pathophysiological link among stearic acid, TNF-α, and MIP-1α/CCL3 in the state of obesity. Here, IRF3 is linked to obesity due to melanocortin 4 receptor deficiency.