The expression levels of the pro-invadopodia regulators cortactin, MMP-2, Src, NWASP (Neural Wiskott-Aldrich syndrome protein), Tks4 (Tyrosine Kinase substrate with 4 SH3 domains), Tks5, and Nck (non-catalytic region of tyrosine kinase adaptor protein), and ion channel (calcium, sodium, and potassium) genes in GBM and non-tumor brain tissue were examined. This evidence concerns the gene NCK1 and glioblastoma.