We re-confirmed the pluripotency of iPSCs using markers OCT4, E-cadherin (E-cad) and Tra-1-60 and verified NPC status through the elevated expression of Pax6, Sox2 and Nestin, with Ki67 as a cell-proliferation marker in NPC (Figure 1A). The gene discussed is POU5F1; the disease is nasopharyngeal carcinoma.