The results indicated that (Figure 3A‐F) HDAC3 siRNA, TRAF5 siRNA or miR‐495‐3p mimics suppressed the proliferation, colony formation ability and tumorigenesis of melanoma cells; the impacts of si‐HDAC3 were abolished by miR‐495‐3p inhibitors. The gene discussed is TRAF5; the disease is melanoma.