Common methods of isolating/enriching CSCs to model sarcoma heterogeneity in vitro include culturing floating three‐dimensional (3D)‐colonies (tumorspheres), cell sorting based on the expression of specific markers (i.e., CD133, ABCG2, CD44, CD184, STRO1, CD117, CD271, or aldehyde dehydrogenase 1), the ability to extrude fluorescent dyes (side populations), or the selective pressure induced by long‐term culturing with chemotherapeutic drugs. Here, CXCR4 is linked to sarcoma.