Comparison of the DNA methylation rates of the iPSC Pos-ESC Neg sites led to the identification of fluctuating methylation regions in six genes, including CSMD1, TMEM132C, TMEM132D, FZD10, DNAH9, and FAM19A5. CSMD1 is known to be a tumor-suppressor gene under the control of DNA methylation in liver cancer and head and neck squamous cell carcinoma [39–41]. Here, CSMD1 is linked to head and neck squamous cell carcinoma.