To tackle these issues, we employed in utero electroporation to knockdown ASD-related genes specifically in layer 2/3 pyramidal neurons of the PFC during development, which was used to examine the roles of Disrupted-in-Schizophrenia-1 (DISC1) gene in synaptic function and schizophrenia-related behaviors49,50. The gene discussed is DISC1; the disease is schizophrenia.