NHP2 and hepatocellular carcinoma: To clarify the effects of NHP2 on HBx-induced HCC, two pairs of chemically synthesized shRNAs (shRNA1 and shRNA2) targeting NHP2 and negative control (sh-NC group) were transfected into PLC/PRF5 hepatoma cells subjected to OverExp-HBx-1 transfection or not, respectively.