In glycolysis, five enzymes involved in the reactions for the conversion of glucose-6-phosphate to pyruvate were hypoacetylated in PCOS granulosa cells, including glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphoglycerate kinase 1 (PGK1), phosphoglycerate mutase 1 (PGAM1), triosephosphate isomerase 1 (TPI1), and alpha-enolase 1 (ENO1). This evidence concerns the gene TPI1 and polycystic ovary syndrome.