Adenovirus serotype 5 (Ad5) expressing immune checkpoint blockers, such as soluble PD-1, anti-PD-1, or anti-PD-L1, has been shown to strongly induce antitumor immune responses and significantly inhibit tumor growth, leading to prolonged survival of tumor-bearing mice (Shin et al., 2013; Tanoue et al., 2017; Kuryk et al., 2019; Zhang et al., 2019). The gene discussed is CD274; the disease is neoplasm.