As immune checkpoints work, the inhibitory signals are mediated by programmed cell death 1 (PD-1) binding to its two specific ligands, programmed cell death ligand 1 (PD-L1), and programmed cell death ligand 2 (PD-L2) expressed on tumor cells, as well as cytotoxic T lymphocyte-associated protein 4 (CTLA-4), binding to B7-1 (CD80), and B7-2 (CD86) molecules on antigen-presenting cells (APCs). The gene discussed is CTLA4; the disease is neoplasm.