EPAS1 DNA numbers (1.4 ± 0.07, 2.10 ± 0.10, 2.41 ± 0.12) in ESCC cancer cell lines KYSE70, KYSE450 and HKESC-1, respectively, are higher when compared with that of nonneoplastic keratinocyte HaCaT (1.01 ± 0.05) cells (Figure 3C). This evidence concerns the gene EPAS1 and esophageal squamous cell carcinoma.