Klip group investigated that nucleotide oligomerization domain (NOD) proteins contribute to glucose uptake in adipose tissue, liver, and muscle cells.[41, 42] Schertzer group indicated that IRF4 was responsible for NOD2‐induced insulin sensitizing and anti‐inflammatory effects during obesity and endotoxemia.[43] In our study, we did not notice any changes in inflammatory genes changed for IRF4 ablation muscle based on RNA‐seq data. The gene discussed is INS; the disease is obesity due to melanocortin 4 receptor deficiency.