To identify the glycosylated proteins that mediate the effects of TSTA3 on ESCC metastasis, we applied LC-MS/MS technology featuring proteomics and N-glycoproteomics to compare the expression level of proteins and glycoproteins in negative control and TSTA3 overexpressed KYSE150 cells. The gene discussed is GFUS; the disease is esophageal squamous cell carcinoma.