The results showed that there was a positive correlation between expression levels of TSTA3 and estimates of copy numbers to some extent (Pearson correlation coefficient = 0.331; P-value = 0.000) (Figure 1D), consistent with the analysis of ESCC tissues without paired normal tissue in TCGA database and the analysis of 27 ESCC cell lines in Cancer Cell Line Encyclopedia (CCLE) (Figure S2D-E). This evidence concerns the gene GFUS and esophageal squamous cell carcinoma.