GFUS and esophageal squamous cell carcinoma: The efficiency of overexpression was confirmed by RT-qPCR and western blot (Figure 2A). Although overexpression of wild-type TSTA3 had no effect on the growth and colony formation of ESCC (Figure 2B-C), TSTA3 exogenous overexpression markedly promoted cell invasion and migration of KYSE150 and KYSE450 (Figure 2D).