Although no obvious and visible metastatic nodules were observed in the liver, the mice injected into ESCC cells with TSTA3-WT exhibited significantly increased lung metastatic nodules compared with the control mice injected into ESCC cells with vector control, which was confirmed by the quantitative analysis and HE staining (P = 0.009) (Figure 4A-C). This evidence concerns the gene GFUS and esophageal squamous cell carcinoma.