The results indicated that there was a significant increase in the concentrations of CCL2 and CXCL8 in the NAFL group when compared with the control group; and the concentrations of CCL3, CCL4, CCL20, CXCL8, and CXCL10 were increased in the NASH group when compared with the control group. This evidence concerns the gene CCL4 and metabolic dysfunction-associated steatohepatitis.