SQSTM1 and acute kidney injury: In our study, by detecting the expression level of LC3, Beclin1 and SQSTM1/p62, we found that autophagy was decreased in I/R-AKI rats when ischemia for 40 min and reperfusion for 24 h, which matched the results of I/R-AKI models in which applied the same ischemia period (40 min) (Nakagawa et al., 2012; Lempiainen et al., 2013; Decuypere et al., 2015).