To verify the relationship between FGFR1 and chemotherapy resistance in gastric cancer, the phosphorylation status of FGFR1 was analyzed in SGC-7901 and BGC-823 cells exposed to 5-fluorouracil (5-FU, 10 μM) for 48 h by Western blot. The gene discussed is FGFR1; the disease is gastric cancer.