A high SFA content increases membrane rigidity, while a high MUFA/PUFA content promotes fluidity.(29) Interestingly, reduced ER membrane fluidity has the potential of mediating ER stress through the regulation of ER calcium channels.(30) Although we have not investigated ER membrane fluidity in the present study, the reduction of SCD activity may cause ER stress via reducing ER membrane fluidity in skeletal muscle of CKD animal and in C2C12 myotubes. This evidence concerns the gene SCD and chronic kidney disease.