Western blot analysis of CRC cells to check the expression status of PNPT1 confirmed that PNPT1 is indeed overexpressed in HCT116p53‐wt,MSI, HCT116p53‐null,MSI, SW480p53‐mut,MSS, and HT29p53‐mut,MSS as compared to SV‐40 transformed CRL1807 normal colon cells (Fig. 6I). This evidence concerns the gene PNPT1 and colorectal carcinoma.