In vivo evaluation of PPBJ significantly reduced the over weight of the body and liver organ weight in HFD induced obese rats and reduced high glucose levels by increasing insulin levels indicated by improved HOMA-IR implies a potential effect of PPBJ on glycemic control and significantly changed the gut microbiota composition with an increased proportion of S. Cerevisiae. Probioticated banana juice tested for lipid profiles, PPBJ significantly decreased the levels of TC and TG in HFD induced hyperlipidemia. Here, INS is linked to hyperlipidemia.