This investigation undertaken to utilize banana peel as appropriate substrate for the formation of pectinase enzyme by SMF method and fermentation of pectinase treated banana juice was carried out by newly isolated strain of S. Cerevisiae and characterized the fermented banana juice for various biochemical and histopathological parameters related to obesity, insulin resistance, oxidative stress, antioxidant activity and high fat diet induced hepatic steatosis. This evidence concerns the gene INS and fatty liver disease.