To evaluate whether the chick embryo may represent a relevant model of PCa metastatic evolution, we retrospectively compared the 3D reconstitution images of metastatic foci seeded by LNCaP, IGR-CaP1, DU145 cells to metastases formed by the NSCLC cell lines (H1975, H1299, A549, H2228 and H1650). The gene discussed is CAP1; the disease is non-small cell lung carcinoma.