Therefore, in order to confirm the impact exerted by pigmentation on BRAFV600E-driven melanoma in vivo, we decided to take advantage of the melanoma-prone Tg(mitfa:BRAFV600E);p53−/−;mitfa−/− transgenic line and to modulate the levels of miR-204 in zebrafish, by means of the miniCoopR vector (Ceol et al., 2011). The gene discussed is MITF; the disease is melanoma.