To generalize our results in other CCA cells-expressing RIPK3, we therefore did a pilot study to screen for the sensitivity to Poly(I:C)/Smac/zVAD-fmk-induced cell death, both RMCCA-1 and HuCCT-1 exhibited sensitivity to Poly(I:C)/Smac/zVAD-fmk treatment, while MMNK1 was completely resistant (data not shown). The gene discussed is RIPK3; the disease is cholangiocarcinoma.