TLR3 and cholangiocarcinoma: In order to explore the sensitivity of CCA cells to TLR3 ligand, Poly(I:C), a commonly used ligand to activate TLR3, we found that none of CCA cell lines that were differentially expressed TLR3 were sensitive to Poly(I:C)-induced cell death both by direct treatment or transfection (Fig. S2A, B).