We observed that both Th17 cells (CD3e+CD4+CD8a−IL17a+) and CD4+RORγt+T cells (CD3e+CD4+CD8a−RORγt+) were more abundant in the CAD mice (Fig. 6c and Additional file 2: Figure S8b), and CAD mice were characterized as having a significant reduction in Treg cells at the siLPL site (Fig. 6d). This evidence concerns the gene CD4 and coronary artery disorder.