ROC analysis revealed that EN-RAGE, sRAGE, EN-RAGE/sRAGE yielded area under the ROC curve (AUC) of 0.88 (95% CI, 0.82–0.94), 0.80 (95% CI, 0.71–0.90) and 0.90 (95% CI, 0.85–0.96), respectively (Fig. 5a), in which EN-RAGE/sRAGE with highest AUC generated 70.14% sensitivity and 96.55% specificity with cutoff value 13.08, revealing that identified EN-RAGE/sRAGE has the highest diagnostic efficacy for AIH. The gene discussed is AGER; the disease is autoimmune hepatitis.