SOD1 and amyotrophic lateral sclerosis: Moreover, Ramachandran plot analysis has also showed the differential distribution of amino acids among favorable, allowed and outlier region require to maintain secondary structure of SOD1 protein suggesting the distorted molecular flexibility and stability of protein of variants at 94 position Such multiple variations at single location may lead to differential clinical phenotypes of ALS based on distribution of amino acid in Ramachandran plot due to varied degree of interactions with downstream molecules.