Quantification of CD8 TIL functional capacity from CON NT, CON + anti-PD-1, ACA NT, and ACA + anti-PD-1 revealed respective stepwise increases in the frequencies of IFNγ+ (Figure S8A) TNFα+, and perforin+ CD8 T cells within the tumor microenvironment (Figure 5D). This evidence concerns the gene PRF1 and neoplasm.